Expressions of CD3 in T cells were 95% and 100% in the BALB/c nude mice and in the BALB/c mice, respectively. We confirmed that the number of CD 3 -positive T lymphocytes in lymph nodes of the nude mice decreased greatly as compared with the BALB/c mice. There were no significant difference between the BALB/c nude mice and the BALB/c mice. Mostly there were two lymph nodes of deep cervical lymph nodes both in the BALB/c nude mice and the BALB/c mice (the constituent ratios were 95% and 100%, respectively). There were significant difference between the BALB/c nude mice and the BALB/c mice. There were two lymph nodes both in the submandible lymph nodes group and in the superficial cervical lymph nodes group (the constituent ratios were 95% and 90%, respectively) in the BALB/c nude mice, but there were four lymph nodes (the constituent ratios were 95% and 90%, respectively) in the BALB/c mice. The anatomical features of the lymph node distribution in the nude mice were mainly found in the neck with relatively higher density. There were, on average, 23 nodes per mouse contained within the large lymph node assembly in the BALB/c nude mouse. Secondly, the differences of T cells and B cells at the lymph node were compared by the expressions of CD 3 and CD 20 immunohistochemistry dyes. Firstly, twenty BALB/c nude mice and twenty BALB/c mice were dissected by using a surgical microscope. To compare the differences of anatomical and histological characteristics of lymph nodes between BALB/c nude mice and BALB/c mice. Absence of T cells in DM might influence the development of DN. At week 4, the increase of type IV collagen in the glomeruli was more prominent in diabetic nude mice than in diabetic WT mice (p<0.05). Correlation analysis showed urine Alb/Cr positively correlated with serum TNF-α level at week 4 (r = 0.588, p<0.01). At week 4, the body weight of nude mice was lower than that of WT mice (14.7 ± 3.15 g vs 17.97 ± 2.85 g, p<0.05) the urine albumin/creatinine ratio (Alb/Cr) of nude mice was higher than that of WT mice (50.96 ± 5.57 mg/mmol vs 41.09 ± 5.79 mg/mmol, p<0.05) the kidney weight to body weight of nude mice was higher than that of WT mice (0.01352 ± 0.00163 vs 0.01173 ± 0.00131, p<0.05). At week 4, the serum TNF- α level of nude mice got to 175.08 ± 46.03 pg/ml (p<0.05, compared with baseline level 80.19 ± 8.46 pg/ml), whereas the TNF- α levels of WT mice was stable. After modeling, there was no difference of blood glucose level between nude mice and WT mice except at week 2 (28.3 ± 4.9 mmol/l vs 23.1 ± 3.9 mmol/l, p<0.01). Serum tumor necrosis factor α (TNF-α), blood glucose, body weight, urine albumin/creatinine ratio and rate of kidney weight to body weight (KW/BW) were measured. Balb/c nude mice and Balb/c wild-type nude (WT) mice were injected with streptozotocin (STZ). To investigate the influence of absence of T cells on DN. However, there is no evidence whether such immune dysfunction can influence the development of DM, especially the development of diabetic nephropathy (DN). Immune dysfunction is very common in diabetes mellitus (DM). Lin, S Xu, P C Huang, Q E Jia, J Y Jia, Z H Wei, L Zheng, Z F Shang, W Y These findings suggest that nude GFP is an ideal model resembling normal nude mice however, GFP expression in various tissues by fluorescence should be considered, as the expression of GFP differs in various organs.ĭevelopment of diabetic nephropathy in nude mice. However, the gene expression levels were relatively less in various tissues compared with B6 GFP mice. The hematological and immune cells of nude GFP were within the range of nude mice. Quantitative real-time PCR (qRT-PCR) was done for evaluating the relative expression of GFP transcripts in few organs of the nude GFP mice. The nude GFP mice were evaluated by imaging, hematological indices, and flow cytometry to compare the proportion of immune T cells. Researchers had developed and characterized transgenic green/red fluorescent protein (GFP/RFP) nude mouse with ubiquitous RFP or GFP expression, but none has evaluated the level of immune cells and expression levels of GFP in this model. Iyer, Srikanth Arindkar, Shailendra Mishra, Alaknanda Manglani, Kapil Kumar, Jerald Mahesh Majumdar, Subeer S Upadhyay, Pramod Nagarajan, Perumal Development and Evaluation of Transgenic Nude Mice Expressing Ubiquitous Green Fluorescent Protein.
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